Efficacy and safety of mitoxantrone hydrochloride liposome injection in treatment of peripheral T-cell lymphomas: a multicenter, non-interventional, ambispective cohort, real-world study (MOMENT) / 白血病·淋巴瘤

Objective:To evaluate the efficacy and safety of mitoxantrone hydrochloride liposome injection in the treatment of peripheral T-cell lymphoma (PTCL) in a real-world setting.Methods:This was a real-world ambispective cohort study (MOMENT study) (Chinese clinical trial registry number: ChiCTR2200062067). Clinical data were collected from 198 patients who received mitoxantrone hydrochloride liposome injection as monotherapy or combination therapy at 37 hospitals from January 2022 to January 2023, including 166 patients in the retrospective cohort and 32 patients in the prospective cohort; 10 patients in the treatment-na?ve group and 188 patients in the relapsed/refractory group. Clinical characteristics, efficacy and adverse events were summarized, and the overall survival (OS) and progression-free survival (PFS) were analyzed.Results:All 198 patients were treated with mitoxantrone hydrochloride liposome injection for a median of 3 cycles (range 1-7 cycles); 28 cases were treated with mitoxantrone hydrochloride liposome injection as monotherapy, and 170 cases were treated with the combination regimen. Among 188 relapsed/refractory patients, 45 cases (23.9%) were in complete remission (CR), 82 cases (43.6%) were in partial remission (PR), and 28 cases (14.9%) were in disease stabilization (SD), and 33 cases (17.6%) were in disease progression (PD), with an objective remission rate (ORR) of 67.6% (127/188). Among 10 treatment-na?ve patients, 4 cases (40.0%) were in CR, 5 cases (50.0%) were in PR, and 1 case (10.0%) was in PD, with an ORR of 90.0% (9/10). The median follow-up time was 2.9 months (95% CI 2.4-3.7 months), and the median PFS and OS of patients in relapsed/refractory and treatment-na?ve groups were not reached. In relapsed/refractory patients, the difference in ORR between patients with different number of treatment lines of mitoxantrone hydrochloride liposome injection [ORR of the second-line, the third-line and ≥the forth-line treatment was 74.4% (67/90), 73.9% (34/46) and 50.0% (26/52)] was statistically significant ( P = 0.008). Of the 198 PTCL patients, 182 cases (91.9%) experienced at least 1 time of treatment-related adverse events, and the incidence rate of ≥grade 3 adverse events was 66.7% (132/198), which was mainly characterized by hematologic adverse events. The ≥ grade 3 hematologic adverse events mainly included decreased lymphocyte count, decreased neutrophil count, decreased white blood cell count, and anemia; non-hematologic adverse events were mostly grade 1-2, mainly including pigmentation disorders and upper respiratory tract infection. Conclusions:The use of mitoxantrone hydrochloride liposome injection-containing regimen in the treatment of PTCL has definite efficacy and is well tolerated, and it is a new therapeutic option for PTCL patients.

Badminton influences on the body constitution of college students / Influências do badminton na constituição corporal dos universitários / Influencias del bádminton en la constitución corporal de los estudiantes universitarios

ABSTRACT Introduction: The physical condition of college students is worsening annually, and the rate of obesity is a factor in alarming rise, influencing the quality of performance in physical education of badminton practice's effects on college students' body composition of college students. Objective: Study badminton practice's effects on college students' body composition. Methods: During a training period of three months, 60 college volunteers were randomly divided into experimental and control groups to practice exercices twice a week for 1.5 hours. The experimental group practiced badminton, while the control group practiced running. Results: The body fat ratio of the experimental group waist-to-hip increased from 38.98% to 33.21%, the waist to hip ratio decreased from 0.83cm to 0.82cm, the vital capacity increased from 2,817ml to 3,111ml, and the resting heart rate decreased from 78 to 75 times/minute. Conclusion: Optimization of body composition and an improvement in body build were statistically observed when implementing badminton training in college students. The promotion of badminton for college students was shown to be superior than running. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: A condição física dos estudantes universitários está piorando anualmente, sendo a taxa de obesidade um fator em alarmante ascensão, influenciando a qualidade do desempenho na educação física dos estudantes universitários. Objetivo: Estudar os efeitos da prática do badminton sobre a composição corporal dos estudantes universitários. Métodos: Durante o período de treinamento de três meses, 60 universitários voluntários foram divididos aleatoriamente em grupo experimental e controle para exercícios praticados duas vezes por semana, com duração de 1,5 horas. O grupo experimental praticou badminton, enquanto o grupo de controle praticou corrida. Resultados: A taxa de gordura corporal do grupo experimental diminuiu de 38,98% para 33,21%, a relação da cintura do quadril diminuiu de 0,83cm para 0,82cm, a capacidade vital aumentou de 2.817ml para 3.111ml, e a frequência cardíaca em repouso diminuiu de 78 para 75 vezes/minuto. Conclusão: A otimização da composição corporal e uma melhoria da constituição corporal foram estatisticamente observadas ao implementar o treinamento de badminton nos universitários. A promoção do badminton para estudantes universitários mostrou-se superior que a corrida.Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: La condición física de los estudiantes universitarios empeora anualmente, siendo la tasa de obesidad un factor en alarmante aumento, influyendo en la calidad del rendimiento en educación física de los estudiantes universitarios. Objetivo: Estudiar los efectos de la práctica del bádminton en la composición corporal de estudiantes universitarios. Métodos: Durante un periodo de entrenamiento de tres meses, 60 voluntarios universitarios fueron divididos aleatoriamente en grupo experimental y grupo de control para ejercicios practicados dos veces por semana, con una duración de 1,5 horas. El grupo experimental practicó bádminton, mientras que el grupo de control practicó atletismo. Resultados: La proporción de grasa corporal del grupo experimental disminuyó del 38,98% al 33,21%, la proporción cintura/cadera disminuyó de 0,83cm a 0,82cm, la capacidad vital aumentó de 2.817ml a 3.111ml, y la frecuencia cardiaca en reposo disminuyó de 78 a 75 veces/minuto. Conclusión: Se observó estadísticamente una optimización de la composición corporal y una mejora de la constitución corporal al aplicar el entrenamiento de bádminton en estudiantes universitarios. Se demostró que la promoción del bádminton entre los estudiantes universitarios era superior a la del atletismo. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Swimming training in college students based on physical fitness / Treinamento de natação em universitários baseado na aptidão física / Entrenamiento de natación en estudiantes universitarios basado en la aptitud física

ABSTRACT Introduction: The current swimming training of college students does not meet the needs for physical fitness, requiring adjustments. Objective: Explore the effects of fitness-based swim training on college students. Methods: Forty volunteers from a university were recruited for the experiment, divided equally and separately by sex into groups A male, A female, B male, and B female. The exercise intensity of male group A and female group A was designed as resting heart rate + 70% * heart rate difference; that of male group B and female group B was designed as resting heart rate + 50% * heart rate difference. The experiment lasted 12 weeks, twice a week. Results: In group A, the forward bending results in the sitting position increased from 13.672 ± 5.8123 cm to 16.368 ± 4.8935 cm after the experiment, and the forward bending results in the sitting position increased from 6.079 ± 4.7637 cm to 18.236 ± 3.9650 cm. The results of both groups were better than those of group B of the same sex. Conclusion: Fitness-based swimming training for college students can improve exercise efficiency by adopting the presented protocol (resting heart rate + 70% * heart rate difference). Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: O treinamento de natação atual dos estudantes universitários não corresponde às necessidades em prol da aptidão física, necessitando de ajustes. Objetivo: Explorar os efeitos do treinamento de natação baseado na aptidão física dos estudantes universitários. Métodos: Foram recrutados 40 voluntários de uma universidade para o experimento, divididos igualmente e separadamente por sexo em grupo A masculino, A feminino, B masculino e B feminino. A intensidade de exercício do grupo masculino A e do grupo feminino A foi projetada como frequência cardíaca em repouso + 70% * diferença de frequência cardíaca; a do grupo masculino B e do grupo feminino B foi projetada como frequência cardíaca em repouso + 50% * diferença de frequência cardíaca. O experimento durou 12 semanas, duas vezes por semana. Resultados: No grupo A, os resultados de flexão para frente na posição sentada aumentaram de 13.672 ± 5.8123 cm para 16.368 ± 4.8935 cm após o experimento, os resultados de flexão para frente na posição sentada aumentaram de 6.079 ± 4.7637 cm para 18.236 ± 3.9650 cm. Os resultados de ambos os grupos foram melhores do que os do grupo B do mesmo sexo. Conclusão: O treinamento de natação baseado na aptidão física dos universitários pode melhorar a eficiência do exercício adotando o protocolo apresentado (frequência cardíaca em repouso + 70% * diferença de frequência cardíaca). Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: El entrenamiento actual de natación de los estudiantes universitarios no se corresponde con las necesidades a favor de la forma física, necesitando ajustes. Objetivo: Explorar los efectos del entrenamiento de natación basado en la condición física en estudiantes universitarios. Métodos: Para el experimento se reclutaron 40 voluntarios de una universidad, divididos equitativamente y por separado según el sexo en el grupo A masculino, A femenino, B masculino y B femenino. La intensidad del ejercicio del grupo masculino A y del grupo femenino A se diseñó como frecuencia cardiaca en reposo + 70% * diferencia de frecuencia cardiaca; la del grupo masculino B y del grupo femenino B se diseñó como frecuencia cardiaca en reposo + 50% * diferencia de frecuencia cardiaca. El experimento duró 12 semanas, dos veces por semana. Resultados: En el grupo A, los resultados de flexión hacia delante en posición sentada aumentaron de 13,672 ± 5,8123 cm a 16,368 ± 4,8935 cm tras el experimento, los resultados de flexión hacia delante en posición sentada aumentaron de 6,079 ± 4,7637 cm a 18,236 ± 3,9650 cm. Los resultados de ambos grupos fueron mejores que los del grupo B del mismo sexo. Conclusión: El entrenamiento de natación basado en la condición física de los estudiantes universitarios puede mejorar la eficacia del ejercicio adoptando el protocolo presentado (frecuencia cardiaca en reposo + 70% * diferencia de frecuencia cardiaca). Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Epidemiological and clinical characteristics of human brucellosis in Enshi Tujia and Miao Autonomous Prefecture of Hubei Province from 2012 to 2019 / 中华地方病学杂志

Objective:To analyze the epidemiological and clinical characteristics of human brucellosis in Enshi Tujia and Miao Autonomous Prefecture (Enshi Prefecture) of Hubei Province, and to provide a basis for prevention and control of local brucellosis.Methods:Using descriptive epidemiological methods, the data of reported cases of brucellosis in Enshi Prefecture from 2012 to 2019 (from the "Enshi Infectious Disease Reporting Information Management System" and the medical record system of hospitals in counties and cities within the jurisdiction of Enshi Prefecture) were collected, and the three distributions (population, time, region distributions) and clinical manifestations of human brucellosis cases were statistically described and analyzed.Results:A total of 78 brucellosis cases were reported in Enshi Prefecture from 2012 to 2019, with an average annual incidence rate of 0.193 5/100 000. Among the 78 cases of brucellosis reported, the sex ratio of men to women was 2.12∶1.00 (53∶25); the age of onset was mainly from 30 to 59 years old, accounting for 75.64% (59/78); the occupation was mainly farmer, accounting for 88.46% (69/78); the main contact animal was sheep, accounting for 80.77% (63/78). There were reported cases of brucellosis throughout the year, and the main onset months were May, June, September, November, and December, accounting for 55.13% (43/78) of the total number. From 2012 to 2019, all counties and cities in Enshi Prefecture except Xianfeng County and Hefeng County had reported cases. Among them, Lichuan City had the most cases, with 55 cases (70.51%). The main clinical manifestations of reported cases of brucellosis were fever, fatigue, hyperhidrosis, and muscle and joint pain, which accounted for 98.72% (77 cases), 89.74% (70 cases), 79.49% (62 cases), and 69.23% (54 cases), respectively.Conclusions:The majority of patients with brucellosis in Enshi Prefecture are young and middle-aged male farmers, with the highest incidence in Lichuan City. Relevant departments should increase the propaganda of brucellosis, increase the people's awareness of disease prevention, and strengthen the prevention and control measures of high-risk groups and regions to reduce the incidence of brucellosis.

Mechanism of period gene regulating renin-angiotensin-aldosterone system in rhythm of blood pressure / 国际脑血管病杂志

Rhythm of blood pressure refers to the circadian variation of blood pressure, which is regulated by clock genes. However, the rhythm disorder of blood pressure increases the risk of stroke. Taking the process of blood pressure regulation as a clue and focusing on the clock gene pathway, this article explores the possible mechanism of period gene regulating renin-angiotensin-aldosterone system in rhythm of blood pressure, so as to provide reference for the in-depth study of the relevant mechanism of rhythm disorder of blood pressure and search for a new target for the primary prevention of cerebrovascular diseases.

Blood pressure rhythm and circadian clock protein expression after cerebral ischemia-reperfusion in spontaneously hypertensive rats / 国际脑血管病杂志

Objective:To investigate the changes of blood pressure and serum circadian clock protein levels after cerebral ischemia-reperfusion in spontaneously hypertensive rat (SHR) and their correlation.Methods:The middle cerebral artery occlusion method was used to prepare the SHR cerebral ischemia-reperfusion model at zero point of Zeitgeber Time (ZT), and the systolic blood pressure within 24 h was continuously monitored after the model was made. The tail vein blood of rats was taken every 3 h, and the changes in serum circadian clock proteins (CLOCK, BMAL1, PER1 and CRY1) levels were detected by enzyme-linked immunosorbent assay. Pearson correlation analysis was used to determine the relationship between blood pressure circadian rhythm pattern and circadian clock protein level fluctuation after cerebral ischemia-reperfusion.Results:In the sham operation group, there were various blood pressure patterns, including dipper (53%), non-dipper (27%), super dipper (13%), and reverse dipper (7%), and the main pattern was dipper. In contrast, the degree of blood pressure disorder in the model group was aggravated, and the non-dipper was the main type, with the proportion as high as 40%. The proportion of super dipper and reverse dipper increased to 27% and 13% respectively; proportion of dipper blood pressure decreased to 20%. The serum level of CLOCK in the model group was relatively stable, while the circadian rhythm of BMAL1, PER1 and CRY1 was significantly changed compared with the sham operation group. Pearson analysis showed that PER1 was negatively correlated with the dipper ( r=-0.565, P=0.002) and super dipper ( r=-0.531, P=0.001) blood pressure patterns, and positively correlated with the non-dipper blood pressure pattern ( r=0.620, P<0.001). Conclusion:The circadian rhythm pattern of blood pressure in SHR after cerebral ischemia-reperfusion was obviously disordered, which was closely associated with the regulation of Per1 gene.

Current research and challenge of human retinal organoid differentiation / 中华实验眼科杂志

The retinal organoid is one of the most widely studied organoids in vitro, which is an "organ in a dish" with a highly similar tissue structure to those in vivo.Since it was first reported, the differentiation methods of retinal organoids have been continously developed to improve the differentiation efficiency and achieve further maturation.Retinal organoids have a broad application prospect as developmental and disease models, as well as seed cells for replacement therapy.However, there are still some problems to be solved, including the universality, effectiveness, heterogeneity of retinal organoid differentiation, as well as the difference between retinal organoids development and the real fetal retinal development.Researchers should investigate the regulational mechanism during organoid development from molecular, genetic, cellular and histological structure levels in order to promote the clinical practices of organoid culture technology.

Circadian clock and ischemic stroke / 国际脑血管病杂志

Almost all life on Earth has a 24 h circadian rhythm. The circadian clock that controls the circadian rhythm is an important regulator of physiology and disease. Disturbance of circadian rhythm can negatively affect physiological homeostasis at the molecular, cellular, organ system, and whole organism levels. The circadian clock rhythm disorders are considered to be involved inmany cardiocerebrovascular diseases, such as ischemic stroke and myocardial infarction. Ischemic stroke is one of the main causes of long-term disability and death worldwide. The incidence is higher in the daytime and lower at night, but the exact mechanism of this time distribution is unclear. This article discusses the role of the circadian clock in stroke pathophysiological mechanism and the specific molecular mechanism of clock gene regulation. It is expected that molecular time can be used or changed to open up new targets for stroke treatment.

Nontraditional lipid indicators and ischemic stroke / 国际脑血管病杂志

Personalized management of ischemic stroke prevention and treatment remains a top priority in the field of neurology. The purpose of this article is to elucidate the role of nontraditional lipid profile and traditional lipids in the pathogenesis and recurrence of acute ischemic stroke in order to provide new indicators for stroke prevention, risk grading and high-risk population screening, and attempt to discuss the potential predictive value of nontraditional lipid indicators.

Relationship between Circadian Rhythm Disorder of Blood Pressure and Ischemic Stroke / 中国医学科学院学报

Hypertension plays an important role in the pathogenesis of stroke,which,however,is only known at the blood pressure level.The relationship between circadian rhythm of blood pressure(especially the circadian rhythm disorder of blood pressure)and stroke has been a hot research topic.This article reviews the concept of circadian rhythm of blood pressure,classification of circadian rhythm disorder of blood pressure,and the relationship of circadian rhythm disorder of blood pressure with ischemic stroke.

c-Fos expression in visual cortex of infant rhesus monkeys with myopia induced by hyperopic defocus / 中华实验眼科杂志

Objective To observe c-Fos expression in visual cortex of infant rhesus monkeys with myopia induced by hyperopic defocus and preliminarily investigate the possibility of visual cortex participating in myopia. Methods Eight SPF grade healthy infant rhesus monkeys aged 20 to 30 days were randomly divided into hyperopic defocused group and control group,4 monkeys for each group. The monkeys in hyperopic defocused group wore -3 D spectacle lenses. The monkeys in control group wore 0 D lenses. The monkeys' refractive error,corneal topography, vitreous chamber depth were measured at the start of lens wear and at 2,4,6,8,12 weeks post-treatment. At 12 weeks post-treatment,the visual cortex tissues were removed for c-Fos protein measurement by immunohistochemistry and Western blot assays. The results were analyzed semiquantitatively to compare the differences of c-Fos expression between hyperopic defocused group and control group. The use and care of the animals complied with Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Commission. This study protocol was approved by Ethic Committee of Zhongshan Ophthalmic Center ( No. 2013-014). Results After 12 weeks'lens wear,the vitreous chamber elongation amplitude of hyperopic defocused group monkeys was more obvious than that of the control group ([0.93±0.24]mm vs. [0.72±0.09]mm;t=2.292,P=0.047). The decrease of hyperopic degrees of hyperopic defocused group monkeys was more obvious than that of the control group ([-3.23± 1.36]D vs. [-1.55±0.52]D;t=-3.273,P=0.006). The eyes of hyperopic defocused group monkeys appeared a remarkable myopic shift after treatment. The number of c-Fos immunoreactive neurons was less in the hyperopic defocused group than that in the control group,with a statistically significant difference between them ([1 843±191]/mm2vs. [2 296±503]/mm2;t=2.381,P=0.041). Western blot assay showed that the optical density of c-Fos protein in the hyperopic defocused group was significantly less than that in the control group (0.50±0.17 vs. 0.99± 0.22;t=-4.982,P<0.01). Conclusions Hyperopic defocus,as an abnormal visual stimulus,can induce the onset of myopia in infant rhesus monkeys and inhibit c-Fos expression in visual cortex. Visual cortex may participate in myopia induced by hyperopic defocus.

Resistance to daunorubicin in acute myeloid leukemia is associated with mutations in the TET2 gene / 安徽医科大学学报

Objective To investigate the effect of leukemia-related genes on drug resistance in patients with acute myeloid leukemia (AML). Methods 74 patients with newly diagnosed AML were selected and 54 leukemia-associated genes of all patients were sequenced by second-generation gene sequencing. The gene with the highest mutation rate was further analyzed in association with resistance to several common chemotherapy medicines in in vitro drug sensitivity assays. In addition, in vitro drug resistance data were compared with the clinical data of patients. Results The TET2 gene was the most frequent mutation among 74 patients with newly diagnosed AML, with 11 positive patients. Among these 11 TET2 positive patients, 9 (81. 82％ ) were resistant to daunorubicin, while only 4 (6. 35％ ) out of 63 TET2 negative patients were resistant to daunorubicin. Besides, there was no significant difference between in vitro resistance rate to daunorubicin and the clinical data of patients. Conclusion TET2 gene mutation is associated with resistance to daunorubicin in AML patients, which may become an important indicator of the therapeutic efficacy of DA regimen.

Chemical constituents from roots of Viburnum setigerum / 中国中药杂志

This experiment was to study the constituents of the roots of Viburnum setigerum through various column chromatographic techniques. Thirteen compounds were obtained and their structures were identified using chemical and spectroscopic methods as (7αH, 8'αH)-4, 4', 8α-trihydroxy-3, 3', 9-trimethoxy-7, 9'-epoxylignan (1), (7αH, 8'αH)-4, 4', 8α, 9-tetrahydroxy-3, 3'-dimethoxy-7, 9'-epoxylignan (2), alashinol G (3), alashinol F (4), (-)-secoisolariciresinol (5), (7R, 7'R, 8R, 8'S)-3, 3'-dimethoxy-7, 7'-epoxylignane -4, 4', 9, 9'-tetraol (6), (7αH, 8αH, 8'βH)-4, 4', 7'α, 9-tetrahydroxy-3, 3'-dimethoxy-7, 9'-epoxylignan (7), loganin (8), dihydroquercetin (9), protocatechuic acid (10), 4-hydroxy-3-methoxy-benzoic acid (11), adoxoside (12), and catechin (13). Compound 1 was a new compound. Compounds 3-7 and 11 were reported from the genus Viburnum for the first time. All compounds were separated from this plant for the first time.

The precise personal management is expected to glaucoma neuroprotection / 中华实验眼科杂志

Glaucoma is a common blinding eye disease mainly characterized by optic nerve damage.Lowing intraocular pressure is still the main managing method of glaucoma up to now.However,although the intraocular pressure is reasonably controlled,optic nerve damage is difficult to recovery and sustainable development in some patients.Therefore,the pathogenesis and prevention and treatment of glaucoma have always been the hot spot of the research of glaucoma in recent years.Even though the big progresses have been made in the experimental research of optical nerve injury and repair,little good evidence has been seen in the clinical management of glaucoma neuroprotection.In recent years,genomics study,stem cells study,molecular biological study,electronic technology application in medical research,especially the rise of big data era laid a good basis for the neuroprotection of glaucoma.Ophthalmologists should focus on new insights into the potential and beyond impact factors in the precise individual therapy of glaucoma neuroprotection.

Effects of Grape Seed Proanthocyanidin on the Transport of Bile Salts in Colon Glandular Cell Caco-2 / 中国药房

OBJECTIVE:To study the effects of grape seed proanthocyanidin(GSP)on the transmembrane transport of sodium glycocholate (GA) and sodium taurocholate (TA) in colon glandular cell Caco-2. METHODS:Caco-2 model was used,and RP-HPLC was conducted to determine the contents of GA and TA in cell culture medium. The test was divided into GSP group, GA group,TA group,GSP+GA group and GSP+TA group,the transport volumes of transporting GA and TA from Transwell apical (AP)side to basolateral(BL)side by Caco-2 cell at 0,2,4,8 h were detected,respectively. RESULTS:The linear ranges of GA and TA were 0.05-1.2 mmol/L(R2=0.9999). With the time passing,transport volumes of GA and TA in BL site in GA group and TA group were sharply increased;while the transport volumes were obviously decreased after adding GSP,with statistical signifi-cance(P<0.01). CONCLUSIONS:GSP has inhibitory effect on the transmembrane transport of GA and TA in Caco-2 cell.

Light exposure resulted in APPswe/PS1 transgenic mice retinal structural and functional disorder / 眼科新进展

Objective To research the functional and structural change in TgAP-PswePS1 transgenic mice after intensive light exposure insult.Methods APPswe/PS1 transgenic mice at the age of 6 months old were grouped for experiments,the transgenic mice were replaced by light insult device for 6 months,while the control mice were kept in normal conditions.After 6 months light exposure,the eyes of control and experimental mice were examined with electroretinography (ERG).The retinal morphology change was investigated with H&E staining.All of the results were quantified and statistically analyzed.Results In the control group,the amplitudes of a and b wave in the rod response were (18.33 ±3.53) μV and (107.58 ± 14.72) μV,while (64.80 ±7.57) μV and (178.76 ± 14.47) μV for the amplitudes of a and b wave in the maximum response;After treated by 6 months of intensive light exposure,in experimental group mice,the amplitudes of a and b wave in the rod response were (17.92 ±4.89) μV and (21.83 ± 5.51) μV;While in the maximum response a striking decrease was detected with a wave (18.23 ±4.44) μV and b wave (24.50 ± 4.49) μV,by compared with control group,the difference were statistical significant (all P ＜ 0.05).Histopathological analysis found significant loss of outer nuclear layer,photoreceptor out segment,whereas controls remained little change in the retina.And the retinal thickness decreased significantly from (181.32 ± 13.47) μm in control group to (102.34 ±9.38) μm after light insults in experimental group,the difference was statistical significant (P =0.017).Conclusion Intensive light exposure can cause the retinal structural and functional disorder in the AP-Pswe/PS1 transgenic mouse.

Progress on diagnosis and treatment of proximal tibiofibular joint dislocation / 中国骨伤

Dislocation of proximal tibiofibular joint(PTFJ) is relatively infrequent in clinic, it can be either isolated or associated with tibia fracture, fibular fracture and ankle injury and so on. Chronic symptomatic PTFJ instability are easily mixed with meniscal tears. It was easily neglected because of the mild clinical presentation and atypical change on radiography. Early diagnosis and treatment are crucial to prevent chronic knee pain and instability. The paper concluded the anatomy, classification, complication, diagnosis, treatment, clinical effect and insufficient of the dislocation of PTFJ, to direct the diagnosis and treatment of proximal tibiofibular joint dislocation in clinical work.

Intensive light exposure inducing choroidal neovascularization in TgAPPswe/PS1 transgenic mice / 眼科新进展

Objective To research the choroidal neovascularization (CNV)in TgAPPswePS1 transgenic mice after intensive light exposure injury.Methods Twenty TgAPPswe/PS1 transgenic mice at the age of 6 months were grouped for experiments.The treated groups of 12 mice were treated by a source of 10 000 lux cool full spectrum light for 6 months,12 hours per day;While the control groups of 8 mice were kept in normal conditions.The mice eyes of the experimental group and control group were examined with HE/Toluidin blue staining,the retinal structure was observed,and the number of CNV was counted.The expression of VEGF and Aβ were examined with immunofluorescence on the retinal pigment epithelial (RPE) flat mount.All of the results were quantified and statistically analyzed.Results After treated by 6 months of intensive light exposure in the experimental group,histopathological analysis has found significant loss of outer nuclear layer/photoreceptor out segment and outer plexiform layer as compared with the control group;At the same time,abnormal hypo-and hyper-pigmentation,vacuoles and disruption in the RPE layer,remarkable CNV were found in the experiment group by Toluidin blue staining,and the incidence of CNV was 18.75％.The VEGF expression domenstrated.a diffusive and deposition pattern along the neovessels which showed a significant increase of (6.59 ± 1.14) fold changes as compared with the control group.The difference was statistical significant (P ＜ 0.05).Then the Aβ deposits were positive expressed in the RPE layers after intensive light exposure treatment,and pathological deposition of Aβ in the RPE showed plaque like displayed by confocal Z-stack microscopy,and the drusenoid Aβ deposits were found alone with the neovessels on the RPE flat mount.The deposition of Aβ protein increased with (6.45 ± 2.93) fold changes as compared with the control group,and the difference was statistical significant.Conclusion CNV with degenerative changes in the outer retina can be induced by intensive light exposure in the APPswe/PS1 transgenic mouse.These results suggest that an Alzheimer's transgenic animal model might be an alternative animal model for CNV if combined with intensive light exposure.

Expression of CXCR7 in Acute Monocytic Leukemia and Its Effects on THP-1 Cell Functions / 中国实验血液学杂志

<p><b>OBJECTIVE</b>To study the expression of stromal cell derived factor-1α (SDF-1α) receptor CXCR7 in acute monocytic leukemia (AML-M5), and its effects on proliferation, apoptosis, invasion of acute monocytic leukemia cell line THP-1.</p><p><b>METHODS</b>CXCR7 protein and mRNA expression levels in THP-1 cells and peripheral blood mononuclear cells (PBMNC) from the newly diagnosed AML-M5 patients and normal individuals were detected by flow cytometry, Western blot and RT-PCR respectively. CCK8, Annexin V/PI double staining and Transwell assay were used to observe the effects of CXCR7 on the proliferation, apoptosis, and invasion of THP-1 cells in vitro.</p><p><b>RESULTS</b>The expression of CXCR7 on immature cell surface of the newly diagnosed AML-M5 patients was significantly higher than that in the control group (P<0.05). CXCR7 was also highly expressed on THP-1 cells surface. The CXCR7 protein and mRNA levels in THP-1 cells and PBMNC of AML-M5 patients were significantly higher than those in the control group (P<0.05). The THP-1 cell proliferation activity was higher in SDF-1α-treated group, but this activity could be inhibited by CXCR7 antibody (P<0.01). CXCR7 antibody did not affect THP-1 cell apoptosis (P>0.05). CXCR7 antibody could inhibit SDF-1α -induced THP-1 cell invasiveness (P<0.01).</p><p><b>CONCLUSION</b>CXCR7 highly expresses in AML-M5 patients and THP-1 cells, and involves in cell proliferation and invasion. The blocking CXCR7 expression can reduce the risk of AML-M5 cell infiltration.</p>

Clinical Study of Acupuncture Treatment for Pulmonary Infection after Acute Cerebral Infarction / 上海针灸杂志

Objective To investigate the clinical therapeutic effect of acupuncture on pulmonary infection after acute cerebral infarction.Methods Seventy patients with pulmonary infection after acute cerebral infarction were randomly allocated to treatment and control groups, 35 cases each. The control group received routine medication and the treatment group, acupuncture in addition. Pre-treatment and post-treatment National Institutes of Health Stroke Scale (NIHSS) scores and clinical pulmonary infection scores (CPIS) were compared between the two groups. The correlation between the NIHSS score and the CPIS score was observed.Results There were statistically significant pre-/post-treatment differences in the NIHSS score and the CPIS score in the two groups (P<0.05,P<0.01). There were statistically significant post-treatment differences in the NIHSS score and the CPIS score between the treatment and control groups (P<0.05). The correlation between the NIHSS score and the CPIS score was low in the treatment group after treatment (r=0.417,P<0.05).Conclusions Acupuncture plus medication is an effective way to treat pulmonary infection after acute cerebral infarction. It can improve the NIHSS score and the CPIS score in the patients.